Extract Blend
Extracting DNA from onions with the use of alcohol?
I just finished a lab of extracting DNA strands from an onion. The onion was blended and mixed with a detergent solution, and then alcohol was poured overtop. Why must the alcohol be ice cold in order for the DNA to be extracted? What does the alcohol actually do in this procedure?
DNA does not dissolve in ethanol (alcohol) and will form a precipitate. In order to maximize how much DNA you get out of your onion, the colder the ethanol needs to be. The colder something is, the less easily things will dissolve in it. If you used something like water, the DNA would have stayed dissolved. The detergent was to break up the cell membranes.
Vegan Chocolate Mint Cake
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